PROW: CD158a

PROW and IWHLDA present the GUIDE on:
CD158a
Authors: R. Biassoni; Christina Bottino
Reviewer: Miguel Lopez-Botet
[GUIDE BAR]

ALTERNATE NAMES FOR CD158a

EB6
MHC class-I specific receptors
p50.1
p58.1

MAJOR LINKS FOR CD158a

No information

FUNCTION BIOCHEMICAL ACTIVITY OF CD158a - No information

CELLULAR FUNCTION OF CD158a

Regulation of NK cell mediated cytolytic activity upon interaction with the appropriate HLA-C alleles.

DISEASE RELEVANCE OF CD158a AND FUNCTION OF CD158a IN INTACT ANIMAL - No information

STRUCTURE MOLECULAR FAMILY FOR CD158a

Families in which CD158a is a member
- CD158a-->Killer Inhibitor Receptor
- CD158a-->p58/p50-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD158a

Type I glycoproteins belonging to the immunoglobulin superfamily
Characterized by two extracellular Ig-like domains
4 members have been characterized at the molecular level**
224 amino acids in extracellular region in all four forms**
Two ITIM motifs characterize the cytoplasmic tail of the inhibitory (p58.1) form.
Four forms differ in cytoplasmic tail, with either 84 or 39 amino acids respectively**

MOLECULAR MASS OF CD158a

CELL TYPE	MW UNREDUCED	MW REDUCED	Comment
NK cells	58 kDa	58 kDa	42 kDa backbone [Inhibiting form (p58.1)]
NK cells	50 kDa	50 kDa	36 kDa cackbone [Activating form (p50.1)]

POST-TRANSCRIPTIONAL MODIFICATION OF CD158a - No information

POST-TRANSLATIONAL MODIFICATION OF CD158a

Several N-linked glycosylation sites
Two tyrosine phosphorylation sites in the long cytoplasmic tails only in the inhibitory (p58) form.

MOLECULAR INTERACTIONS PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD158a - No information

SUBSTRATES FOR CD158a - No information

ENZYMES WHICH MODIFY CD158a - No information

LIGANDS FOR CD158a AND MOLECULES ASSOCIATED WITH CD158a

MOLECULE	COMMENT
HLA-Cw4 and related alleles (HLA-Cw2, -Cw5 and -Cw6) and related allotypes displaying Asn 77 and Lys 80.	Ligand
SHP-1	Tyrosine phosphatase SHP-1 binds to tyrosine phosphorylated ITIM in the cytoplasmic tail**

EXPRESSION MAIN CELLULAR EXPRESSION OF CD158a

p58.1 and p50.1 molecules are expressed by NK cell subsets and rare T cells (either CD3/TCRa/b+ or CD3/TCRg/d+ T lymphocytes).*

AUTHOR'S ADDITIONAL INSIGHTS ON CD158a

This CD includes two different molecules (p58.1 and p50.1) with divergent functional effects (i.e. inhibition and activation, respectively), which are presumably encoded by different genes of the same family**

REAGENTS CD158a-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
EB6	A Van Aghtoven, Marseille
HP-3E4	M Lopez-Botet, Madrid	Note HP-3E4 mAb reacts with other members of the family (i.e. p50.3)

SELECTION OF OTHER CD158a-SPECIFIC REFERENCE MAB - No information

SELECTED REFERENCES ON CD158a

REVIEWS

1. Moretta A,Bottino C,Vitale M,Pende D,Biassoni R,Mingari MC,Moretta L Receptors for HLA class-I molecules in human natural killer cells. Annu Rev Immunol 1996 14:619 PubMed

2. NK cells, MHC class I antigens and missing self. Immunological Reviews, Vol.155,pp. 5-221 1997

PRIMARY CITATIONS

1. Moretta A,Bottino C,Pende D,Tripodi G,Tambussi G,Viale O,Orengo A,Barbaresi M,Merli A,Ciccone E,et al Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition. J Exp Med 1990 172:1589 PubMed

2. Melero I,Salmeron A,Balboa MA,Aramburu J,Lopez-Botet M Tyrosine kinase-dependent activation of human NK cell functions upon stimulation through a 58-kDa surface antigen selectively expressed on discrete subsets of NK cells and T lymphocytes. J Immunol 1994 152:1662 PubMed

WWW RESOURCES

* indicates amended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99 mpr@mail.nih.gov