• L-selectin
• LAM-1
• LECAM-1
• Leu-8
• MEL-14
• TQ-1

MAJOR LINKS FOR CD62L  

• NCBI LocusLink Record: 6402
• Mendelian Inheritance in Man (OMIM): 153240
• SwissProt annotated protein record: P14151

CELLULAR FUNCTION OF CD62L  

• CD62L mediates lymphocyte homing to high endothelial venules of peripheral lymphoid tissue and leukocyte rolling on activated endothelium at inflammatory sites*

DISEASE RELEVANCE OF CD62L AND FUNCTION OF CD62L IN INTACT ANIMAL  

• In CD62L-deficient mice, lymphocytes do not bind to peripheral lymph node high endothelial venules*
• In CD62L-deficient mice, there are significant defects in leukocyte rolling and neutrophil, monocyte, and lymphocyte migration into tissues in response to inflammatory stimuli*

• Families in which CD62L is a member
  • CD62L-->selectin-->c-type lectin

MOLECULAR STRUCTURE OF CD62L  

• 334 amino acids
• Extracellular region: 279 aa
• Transmebrane region: 23 aa
• Intracellular region: 17 aa*
• One C-type lectin domain, one EGF-like domain, and two SCR domains are present from the N-terminus in the extracellular region*

MOLECULAR MASS OF CD62L  

POST-TRANSCRIPTIONAL MODIFICATION OF CD62L   - Unknown

POST-TRANSLATIONAL MODIFICATION OF CD62L  

• Seven possible N-linked glycosylation sites are present in the extracellular region
• CD62L is endoproteolytically cleaved from the cell surface at membrane-proximal extracellular site (K268-S269) which is 11 amino acids away from the membrane-spanning domain*

SUBSTRATES FOR CD62L   - Unknown

ENZYMES WHICH MODIFY CD62L   - Unknown

LIGANDS FOR CD62L AND MOLECULES ASSOCIATED WITH CD62L  

• Note 1 - CD62L binds a series of heavily glycosylated, fucosylated, sulfated sialylated glycoproteins that included CD34, glycam-1 and MAdCam-1**

• Most peripheral blood B cells, T cells, monocytes and granulocytes express CD62L
• Some NK cells express CD62L
• Some spleen lymphocytes, bone marrow lymphocytes, bone marrow myeloid cells and thymocytes express CD62L
• Certain hematopoietic malignant cells express CD62L

SELECTION OF OTHER CD62L-SPECIFIC REFERENCE MAB  

REVIEWS

PRIMARY CITATIONS

1. Chen A,Engel P,Tedder TF Structural requirements regulate endoproteolytic release of the L- selectin (CD62L) adhesion receptor from the cell surface of leukocytes. J Exp Med 1995 182:519 PubMed

2. Griffin JD,Spertini O,Ernst TJ,Belvin MP,Levine HB,Kanakura Y,Tedder TF Granulocyte-macrophage colony-stimulating factor and other cytokines regulate surface expression of the leukocyte adhesion molecule-1 on human neutrophils, monocytes, and their precursors. J Immunol 1990 145:576 PubMed

3. Migaki GI,Kahn J,Kishimoto TK Mutational analysis of the membrane-proximal cleavage site of L- selectin: relaxed sequence specificity surrounding the cleavage site. J Exp Med 1995 182:549 PubMed

4. Spertini O,Freedman AS,Belvin MP,Penta AC,Griffin JD,Tedder TF Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells. Leukemia 1991 5:300 PubMed

5. Spertini O,Luscinskas FW,Gimbrone MA Jr,Tedder TF Monocyte attachment to activated human vascular endothelium in vitro is mediated by leukocyte adhesion molecule-1 (L-selectin) under nonstatic conditions. J Exp Med 1992 175:1789 PubMed

6. Spertini O,Luscinskas FW,Kansas GS,Munro JM,Griffin JD,Gimbrone MA Jr,Tedder TF Leukocyte adhesion molecule-1 (LAM-1, L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte adhesion. J Immunol 1991 147:2565 PubMed

7. Tedder TF,Isaacs CM,Ernst TJ,Demetri GD,Adler DA,Disteche CM Isolation and chromosomal localization of cDNAs encoding a novel human lymphocyte cell surface molecule, LAM-1. Homology with the mouse lymphocyte homing receptor and other human adhesion proteins. J Exp Med 1989 170:123 PubMed

8. Tedder TF,Matsuyama T,Rothstein D,Schlossman SF,Morimoto C Human antigen-specific memory T cells express the homing receptor (LAM- 1) necessary for lymphocyte recirculation. Eur J Immunol 1990 20:1351 PubMed

9. Tedder TF,Penta AC,Levine HB,Freedman AS Expression of the human leukocyte adhesion molecule, LAM1. Identity with the TQ1 and Leu-8 differentiation antigens. J Immunol 1990 144:532 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov